The Midwest Women's Lacrosse Conference (MWLC) was founded in 2010 and began play during the spring of 2011.
The conference is comprised of 14 NCAA Division III institutions located throughout the Midwest including Aurora (Aurora, Ill.), Augsburg (Minneapolis, Minn.), Beloit (Beloit, Wis.), Benedictine (Lisle, Ill.), Concordia Wisconsin (Mequon, Wis.), Concordia Chicago (River Forest, Ill.), Cornell College (Mt. Vernon, Iowa), Hamline (Saint Paul, Minn.), Illinois Institute of Technology (Chicago, Ill.), Loras (Dubuque, Iowa), Marian (Fond du Lac, Wis.), Monmouth (Monmouth, Ill.), Northwestern (St. Paul, Minn.) and Wartburg (Waverly, Iowa).
Adrian, Albion and Trine left the Midwest Women's Lacrosse Conference after the 2012 season when the Michigan Intercollegiate Athletic Association (MIAA) began sponsoring women's lacrosse. The CCIW and HCAC institutions left the MWLC after the 2014 season along with Dubuque and Fontbonne.
Cornell, Illinois Institute of Technology, and Wartburg began conference play in the spring of 2015, joining members Aurora, Beloit, Benedictine, Concordia Wisconsin, and Loras. Augsburg and Concordia Chicago joined the spring of 2016, while Hamline and Monmouth joined the MWLC ranks in the spring of 2017. Marian and Northwestern became the league's newest members in the spring of 2019.